DNA segregation during the cell cycle of Escherichia coli

An analysis of intracellular positions of fluorescently labelled DNA regions

Thesis of Marco Roos, written to receive the doctor's degree (Ph D) in biology at the University of Amsterdam on the authority of Rector Magnificus prof. dr. J. J. M. Franse, after defending the thesis in public for a committee assembled by the college of promotions on thursday the fifth of July 2001 at 11.00 hours


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Table of contents

Ch. 1.IntroductionPage 1
2.Cellular localisation of oriC during the cell cycle of Escherichia coli as analysed by fluorescent in situ hybridisation31
Published in Biochimie 81: 797-802 (1999)
3.The replicated ftsQAZ and minB chromosomal regions of Escherichia coli segregate on average in line with nucleoid movement45
Published in Molecular Microbiology 39(3): 633-640 (2001)
4.Experiments on movement of DNA regions in Escherichia coli evaluated by computer simulation65
Published in Biochimie 83: 67-74 (2001)
5.Assumptions revisited85
6.DNA organisation and DNA segregation in Escherichia coli
General discussion
105
Summary123
DNA in beweging
Samenvatting voor de leek (summary for laymen in Dutch)
127
Dankwoord
Acknowledgements (largely in Dutch)
123
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